Agar-agar is a polysaccharide obtained from marine seaweed of the Rhodophycaeae family through an
extraction process that results in a fine homogeneous powder. Bacteriological agar is the essential
gelling agent in the preparation of culture media for microbial growth. The main advantage of this special
type of agar is the absence of contaminants that can inhibit the optimal growth of microorganisms, along
with its exceptional transparency and lot to lot consistency. This type of agar undergoes strict controls to
guarantee its superior quality and excellent consistent performance.
Thanks to its high degree of purity it gives clear aqueous solutions at the concentrations used in culture
media (13-15 g/l).
TYPICAL ANALYSIS
- Appearance of powder White-yellowish, fine, homogeneous
hygroscopic powder - Appearance of 1.3% solution Yellowish, limpid
- Water absorbtion (5 g/100ml) > 25 ml
- Sieve analysis (ASTM) – over sieve
60 mesh < 5%
325-400 mesh < 10%
% H20 < 10% - Ash < 5%
- BEFORE AUTOCLAVING:
- Clarity 1,5% solution < 15 NTU
- Colorimetry 450 nm 1,5% solution < 0,250 Nmu
pH 1,5% solution 6.0 – 7.5 - Gel strenght (Nikkan) > 750 g/cm²
- Gelling temperature (1.5% solution) 30 – 40 °C
- Melting temperature (1.5% solution) 80 – 90 °C
- Performances in microbiological culture media passes test
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