Cled Medium
Suspend the components, dehydrated powder, in water (36.13 grams in 1000 ml of purified/distilled water). The medium is boiled for a few seconds until the ingredients are completely dissolved. Mix well and finally sterilize by autoclaving at 121°C for 15 minutes.
Cool to 50-55°C and dispense aseptically into sterile Petri dishes. Date the medium and assign it a lot number.
Cled agar composition |
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Ingredients | gram/liter | Ingredients | gram/liter |
Balanced Peptone | 4.0 g | Tryptone | 4.0 g |
Beef extract | 3.0 g | Lactose | 10.0 g |
L-Cystine | 0.128 g | Bromothymol Blue | 0.02 g |
Agar | 15.0 g | Final pH | 7.3 +/- 0.2 |
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