Cled Medium

Cled Medium

Cled agar (Cystine Lactose Electrolyte Deficient ) is differential, non selective culture medium used for the isolation, enumeration and differentiation of urinary microorganisms. It promotes the growth of urinary pathogens, but prevents excessive swarming of Proteus species due to its lack of electrolytes.

The medium allows quantitative determination of urinary pathogens including Proteus when calibrated loops are used for inoculation.

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Cled Medium

Suspend the components, dehydrated powder, in water (36.13 grams in 1000 ml of purified/distilled water). The medium is boiled for a few seconds until the ingredients are completely dissolved. Mix well and finally sterilize by autoclaving at 121°C for 15 minutes.

Cool to 50-55°C and dispense aseptically into sterile Petri dishes. Date the medium and assign it a lot number.

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