E.C.O.A. Agar
Chromogenic Substrates 180.0 mg
Selective compounds 26.0 mg
K ANAMYCIN S ELECTIVE SUPPLEMENT (vial contents for 500ml of medium)
Kanamycin Sulphate 10 mg
E.C.O.A.GAR (ready to use plates)
Peptones 28.0
Sodium chloride 5.0
Glucose 1.0
Emulsifying agents 5.7
Phosphate buffer 5.0
Agar 15.0
Chromogenic Substrates 180.0 mg
Selective compounds 26.0 mg
Kanamycin Sulphate 20 mg
Distilled water 1000 ml
Directions
Suspend 30 g in 500ml of cold distilled water. Heat to boiling to dissolve completely. Sterilise by
autoclaving at 121°C for 15 minutes. Cool to 50°C and add the contents of one vial of Kanamycin
Selective Supplement reconstituted with 5ml of sterile distilled water. Mix well to suspend the
flocculent blue precipitate and distribute into sterile Petri dishes.
Final pH 7.2 ± 0.2
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